The Human Renin ELISA Kit from AssayGenie is a cutting-edge tool designed for the precise measurement of renin levels in human samples. Renin is a key enzyme in the Renin-Angiotensin System (RAS), which plays a critical role in regulating blood pressure and fluid balance in the body. This ELISA kit offers superior sensitivity and specificity, ensuring accurate and reproducible results in serum, plasma, and cell culture supernatants.
Its easy-to-use format makes it suitable for a wide range of research applications, including studies on hypertension, kidney diseases, and cardiovascular disorders. By accurately quantifying renin levels, researchers can gain valuable insights into the pathophysiology of various diseases and potentially identify new therapeutic targets. Trust the Human Renin ELISA Kit from AssayGenie for reliable and impactful research outcomes.
Product Name:
Human REN (Renin) ELISA Kit
SKU:
HUES01403
Target:
Human REN (Renin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human REN. You can calculate the concentration of Human REN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-100
98-113
91-106
Average (%)
92
105
98
1:4
Range (%)
85-97
95-112
91-105
Average (%)
91
103
97
1:8
Range (%)
85-96
96-113
90-105
Average (%)
90
104
96
1:16
Range (%)
85-100
95-106
88-105
Average (%)
91
101
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
102
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
94-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.51
320.61
689.84
101.2
302.1
696.38
Standard deviation
4.77
13.43
29.04
6.43
17.82
34.61
C V (%)
5.16
4.19
4.21
6.35
5.9
4.97
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human REN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human REN in samples. No significant cross-reactivity or interference between Human REN and analogues was observed.
