The Human Ribonuclease L (RNaseL) ELISA Kit from Assay Genie is specifically designed to accurately quantify levels of Ribonuclease L in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Ribonuclease L is a key enzyme involved in the innate immune response, playing a critical role in antiviral defense mechanisms and the regulation of cell proliferation and apoptosis. Dysregulation of Ribonuclease L activity has been implicated in various diseases including viral infections, autoimmune disorders, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With the Human Ribonuclease L (RNaseL) ELISA Kit, researchers can accurately measure Ribonuclease L levels, providing valuable insights into immune function and disease pathology. Trust Assay Genie for high-quality ELISA kits that deliver precise and meaningful results for your research needs.
Product Name:
Human Ribonuclease L (RNASEL) ELISA Kit
Product Code:
HUDL02550
Size:
96 Assays
Target:
RNASEL
Synonyms:
Rnase-L, RNS4, PRCA1, 2',5'-Oligoisoadenylate Synthetase-Dependent, Prostate Cancer 1, 2-5A-dependent ribonuclease
Detection Method:
Sandwich
Range:
15.6-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
