Human Ribonucleotide Reductase M1 (RRM1) ELISA Kit
The Human Ribonucleotide Reductase M1 (RRM1) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of RRM1 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for researchers studying the role of RRM1 in various diseases and conditions.Ribonucleotide reductase is a key enzyme involved in DNA synthesis and repair, making it a critical target for cancer therapy. RRM1, as a subunit of ribonucleotide reductase, plays a crucial role in regulating DNA synthesis and cell proliferation.
Aberrant expression of RRM1 has been implicated in cancer progression and resistance to chemotherapy, highlighting the importance of studying its levels in disease pathogenesis.With its high sensitivity and specificity, the Human Ribonucleotide Reductase M1 (RRM1) ELISA Kit is a valuable tool for researchers looking to explore the molecular mechanisms underlying cancer and other diseases, ultimately leading to the development of novel therapeutic strategies.
Product Name:
Human Ribonucleotide Reductase M1 (RRM1) ELISA Kit
Product Code:
HUDL02569
Size:
96 Assays
Target:
RRM1
Synonyms:
R1, RIR1, RR1, Ribonucleoside-diphosphate reductase large subunit
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
