Human RPS6K beta1 (Ribosomal Protein S6 Kinase Beta 1) CLIA Kit
The Human RPS6K Beta1 (Ribosomal Protein S6 Kinase Beta-1) CLIA Kit is a reliable tool for the sensitive detection of RPS6K Beta1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring consistent and reproducible results for a variety of research applications.RPS6K Beta1 is a key protein involved in cell growth and proliferation, playing a crucial role in signaling pathways related to cell survival and growth regulation.
Dysregulation of RPS6K Beta1 has been linked to various diseases including cancer, diabetes, and neurodegenerative disorders, making it an important biomarker for investigating these conditions and exploring potential therapeutic strategies.Overall, the Human RPS6K Beta1 CLIA Kit provides researchers with a valuable tool for studying the role of RPS6K Beta1 in health and disease, offering a reliable platform for precise and accurate quantification of this important protein.
Product Name:
Human RPS6K beta1 (Ribosomal Protein S6 Kinase Beta 1) CLIA Kit
SKU:
HUES00695
Target:
Human RPS6K beta1 (Ribosomal Protein S6 Kinase Beta 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human RPS6Kβ1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RPS6Kβ1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RPS6Kβ1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human RPS6Kβ1. You can calculate the concentration of Human RPS6Kβ1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
84-98
84-96
Average (%)
102
91
90
1:4
Range (%)
98-115
98-113
99-116
Average (%)
105
104
107
1:8
Range (%)
98-111
93-107
93-110
Average (%)
105
99
100
1:16
Range (%)
87-103
92-104
101-116
Average (%)
94
97
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
96
EDTA plasma (n=5)
97-108
102
Cell culture media (n=5)
89-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.09
293.02
771.96
112.1
296.47
736.9
Standard deviation
13.97
25.84
57.82
12.29
27.42
68.16
C V (%)
12.69
8.82
7.49
10.96
9.25
9.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human RPS6Kβ1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RPS6Kβ1 in samples. No significant cross-reactivity or interference between Human RPS6Kβ1 and analogues was observed.
