Human S100 Calcium Binding Protein P (S100P) ELISA Kit
The Human S100 Calcium Binding Protein P (S100P) ELISA Kit is specifically designed for the accurate measurement of S100P levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and consistent results, making it an invaluable tool for a variety of research applications.S100P is a prominent member of the S100 protein family known for its calcium-binding properties and involvement in numerous cellular processes. It plays a critical role in regulating cell proliferation, differentiation, and migration, making it a key player in various physiological and pathological conditions.
With the Human S100 Calcium Binding Protein P (S100P) ELISA Kit, researchers can assess S100P levels in biological samples with precision, providing valuable insights into its functions and potential implications in diseases such as cancer, inflammatory disorders, and neurodegenerative diseases. Unlock the potential of your research with this innovative ELISA kit.
Product Name:
Human S100 Calcium Binding Protein P (S100P) ELISA Kit
Product Code:
HUDL02593
Size:
96 Assays
Target:
S100P
Synonyms:
S100-P, MIG9, S100-E, Migration-inducing gene 9 protein
Detection Method:
Sandwich
Range:
78.1-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
