Human S100A8 (S100 Calcium Binding Protein A8) CLIA Kit
The Human S100A8 (S100 Calcium Binding Protein A8) CLIA Kit is designed for precise and reliable detection of S100A8 levels in human serum, plasma, and cell culture supernatants. This highly sensitive and specific kit provides accurate results, making it suitable for various research applications.S100A8, also known as S100 Calcium Binding Protein A8, is a crucial protein involved in inflammation and immune response. It plays a significant role in various diseases such as cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing potential treatments.
This CLIA kit offers a simple and efficient method for measuring S100A8 levels, allowing researchers to gather important data for their studies. With its high sensitivity and specificity, this kit is a valuable tool in the field of biomedical research.
Product Name:
Human S100A8 (S100 Calcium Binding Protein A8) CLIA Kit
SKU:
HUES00741
Target:
Human S100A8 (S100 Calcium Binding Protein A8)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human S100A8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human S100A8. You can calculate the concentration of Human S100A8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
89-103
89-100
Average (%)
92
96
95
1:4
Range (%)
93-109
92-107
95-108
Average (%)
100
99
100
1:8
Range (%)
91-105
93-108
98-113
Average (%)
96
100
104
1:16
Range (%)
95-108
96-110
94-109
Average (%)
102
104
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
406.61
921.22
3331.98
427.74
932.86
3086.39
Standard deviation
35.66
91.11
371.52
37.43
76.59
228.39
C V (%)
8.77
9.89
11.15
8.75
8.21
7.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human S100A8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100A8 in samples. No significant cross-reactivity or interference between Human S100A8 and analogues was observed.
