Human SCCA2 (Squamous Cell Carcinoma Antigen 2) CLIA Kit
The Human SCCA2 (Squamous Cell Carcinoma Antigen 2) CLIA Kit is a cutting-edge diagnostic tool designed for the precise detection of SCCA2 levels in human samples. This kit offers unparalleled sensitivity and specificity, ensuring accurate and consistent results for various research applications.SCCA2 is a key biomarker associated with squamous cell carcinoma, a type of skin cancer. Elevated levels of SCCA2 are linked to the progression and prognosis of this disease, making it a valuable marker for monitoring cancer development and treatment response.
With its advanced technology and reliable performance, the Human SCCA2 CLIA Kit is a valuable tool for researchers and clinicians studying squamous cell carcinoma and exploring potential therapeutic interventions. Trust in this kit to provide you with the data needed to advance your research and improve patient care.
Product Name:
Human SCCA2 (Squamous Cell Carcinoma Antigen 2) CLIA Kit
SKU:
HUES00963
Target:
Human SCCA2 (Squamous Cell Carcinoma Antigen 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SCCA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCCA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SCCA2. You can calculate the concentration of Human SCCA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-105
93-106
91-106
Average (%)
98
98
97
1:4
Range (%)
95-111
85-101
100-120
Average (%)
102
92
109
1:8
Range (%)
84-95
101-115
95-107
Average (%)
90
108
101
1:16
Range (%)
95-107
97-108
89-100
Average (%)
101
102
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
97
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
98-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.93
296.98
825.42
103.66
315.96
844.66
Standard deviation
11.51
31.0
73.54
9.32
37.03
90.89
C V (%)
11.63
10.44
8.91
8.99
11.72
10.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SCCA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SCCA2 in samples. No significant cross-reactivity or interference between Human SCCA2 and analogues was observed.
