Human sCD163 (Soluable Cluster of Differentiation 163) ELISA Kit
The Human SCD163 (Soluable Cluster of Differentiation 163) ELISA Kit is specially designed for the accurate measurement of SCD163 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and reliable results for various research applications.SCD163, also known as CD163, is a key protein involved in the regulation of inflammation and immune responses. It plays a crucial role in conditions such as sepsis, liver disease, and cardiovascular disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With its high-performance features, the Human SCD163 ELISA Kit is an essential tool for researchers looking to explore the role of SCD163 in health and disease, offering precise and accurate measurement of this important protein level in human samples.
Product Name:
Human sCD163 (Soluable Cluster of Differentiation 163) ELISA Kit
SKU:
HUES01323
Target:
Human sCD163 (Soluable Cluster of Differentiation 163)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sCD163. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sCD163 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sCD163, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sCD163. You can calculate the concentration of Human sCD163 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
94-110
88-103
Average (%)
104
101
95
1:4
Range (%)
91-105
82-92
88-100
Average (%)
97
87
94
1:8
Range (%)
91-103
83-93
86-101
Average (%)
96
88
92
1:16
Range (%)
87-100
82-98
86-101
Average (%)
93
89
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.07
10.69
42.24
4.86
11.16
43.73
Standard deviation
0.35
0.52
2.1
0.32
0.57
1.64
C V (%)
6.9
4.86
4.97
6.58
5.11
3.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sCD163 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sCD163 in samples. No significant cross-reactivity or interference between Human sCD163 and analogues was observed.
