The Human SDC3 (Syndecan-3) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Syndecan-3 levels in human samples such as serum, plasma, and cell culture supernatants. Syndecan-3 is a cell surface proteoglycan involved in cell adhesion, migration, and signaling, making it a key player in various physiological and pathological processes.This kit offers reliable and reproducible results, making it ideal for a wide range of research applications focusing on Syndecan-3 function and its implications in various diseases such as cancer, inflammation, and neuronal disorders.
By accurately measuring Syndecan-3 levels, researchers can gain valuable insights into its role in disease progression and potentially identify new diagnostic and therapeutic targets. Overall, the Human SDC3 CLIA Kit is a valuable tool for researchers looking to study Syndecan-3 biology and its impact on human health.
Product Name:
Human SDC3 (Syndecan 3) CLIA Kit
SKU:
HUES00719
Target:
Human SDC3 (Syndecan 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SDC3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDC3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SDC3. You can calculate the concentration of Human SDC3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
91-108
100-115
Average (%)
103
99
108
1:4
Range (%)
94-107
89-100
97-109
Average (%)
100
94
103
1:8
Range (%)
98-115
91-105
99-113
Average (%)
106
98
106
1:16
Range (%)
95-108
87-101
97-110
Average (%)
102
92
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
99
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
364.45
1052.43
3466.12
396.88
1093.04
3176.84
Standard deviation
39.32
124.61
237.43
33.26
84.6
336.75
C V (%)
10.79
11.84
6.85
8.38
7.74
10.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SDC3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SDC3 in samples. No significant cross-reactivity or interference between Human SDC3 and analogues was observed.
