The Human Sonic Hedgehog (SHH) Homolog CLIA Kit is specially designed for precise and efficient detection of SHH levels in human biological samples including serum, plasma, and cell culture supernatants. This kit is known for its superior sensitivity and specificity, guaranteeing consistent and dependable results, making it the perfect choice for a variety of research applications.SHH, a key signaling molecule in the Hedgehog pathway, plays a vital role in various biological processes such as cell differentiation, tissue growth, and organ development.
Dysregulation of SHH signaling is associated with several diseases including cancer, birth defects, and neurological disorders, highlighting its significance as a biomarker for diagnostic and therapeutic development.Trust the Human SHH Homolog CLIA Kit to provide accurate and reliable data for your research needs. Order now and unlock the potential of studying SHH biology with precision and confidence.
Product Name:
Human SHH (Hedgehog Homolog, Sonic) CLIA Kit
SKU:
HUES00553
Target:
Human SHH (Hedgehog Homolog, Sonic)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SHH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SHH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SHH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SHH. You can calculate the concentration of Human SHH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
91-105
85-99
Average (%)
90
98
91
1:4
Range (%)
92-105
96-107
101-118
Average (%)
98
101
108
1:8
Range (%)
86-98
94-106
91-104
Average (%)
93
100
99
1:16
Range (%)
104-119
91-105
100-118
Average (%)
110
98
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-117
108
EDTA plasma (n=5)
87-99
93
Cell culture media (n=5)
100-115
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.59
142.26
487.44
51.44
143.36
491.39
Standard deviation
5.35
15.02
51.77
6.22
11.76
44.62
C V (%)
10.17
10.56
10.62
12.09
8.2
9.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SHH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SHH in samples. No significant cross-reactivity or interference between Human SHH and analogues was observed.
