The Mouse SHH (Hedgehog Homolog Sonic) ELISA Kit is specifically designed for the accurate detection of Sonic Hedgehog levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research purposes. Sonic Hedgehog is a key signaling protein involved in embryonic development, cell differentiation, and tissue regeneration. Dysregulation of the SHH pathway has been linked to various diseases, including cancer, developmental disorders, and neurological conditions. Therefore, this ELISA kit is an invaluable tool for studying the role of Sonic Hedgehog in these conditions and exploring potential therapeutic interventions. With its user-friendly protocol and reliable performance, the Mouse SHH ELISA Kit from Assay Genie is an essential resource for researchers investigating the intricate mechanisms of Sonic Hedgehog signaling in mouse model systems.
Product Name:
Mouse SHH (Hedgehog Homolog, Sonic) ELISA Kit
SKU:
MOES01131
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
