The Human SHH (Sonic Hedgehog Homolog) ELISA Kit is specifically designed for the precise measurement of Sonic Hedgehog Homolog levels in human samples including serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research purposes.Sonic Hedgehog Homolog is a key signaling protein that plays a crucial role in embryonic development and tissue regeneration. Dysregulation of SHH signaling has been implicated in a variety of diseases, including cancer, neurological disorders, and developmental anomalies.
By accurately measuring SHH levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially identify new treatment strategies.Overall, the Human SHH ELISA Kit from AssayGenie provides a reliable tool for investigating the role of Sonic Hedgehog Homolog in various physiological and pathological processes, offering researchers a valuable resource for advancing their studies in the field of molecular biology and biomedicine.
Product Name:
Human SHH (Hedgehog Homolog, Sonic) ELISA Kit
SKU:
HUES02054
Target:
Human SHH (Hedgehog Homolog, Sonic)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SHH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SHH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SHH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SHH. You can calculate the concentration of Human SHH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
84-97
98-110
Average (%)
102
90
104
1:4
Range (%)
87-99
83-93
81-93
Average (%)
93
88
88
1:8
Range (%)
89-101
81-94
86-100
Average (%)
94
87
93
1:16
Range (%)
91-104
79-90
82-92
Average (%)
97
85
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
265.23
629.21
1862.2
280.01
601.38
1709.28
Standard deviation
13.39
32.09
59.4
15.57
26.52
68.03
C V (%)
5.05
5.1
3.19
5.56
4.41
3.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SHH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SHH in samples. No significant cross-reactivity or interference between Human SHH and analogues was observed.
