Human SIGLEC10 (Sialic Acid Binding Ig Like Lectin 10) CLIA Kit
The Human SIGLEC10 (Sialic Acid Binding Ig-like Lectin 10) CLIA Kit is designed for the precise measurement of SIGLEC10 levels in human serum, plasma, and cell culture supernatants. This cutting-edge kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results that are invaluable for various research purposes.SIGLEC10 is a key protein that binds to sialic acid, playing a critical role in immune responses and inflammation regulation. Dysregulation of SIGLEC10 has been implicated in a variety of diseases, including autoimmune disorders, cancer, and infectious diseases, making it a promising biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and reliable performance, the Human SIGLEC10 CLIA Kit is an indispensable tool for researchers studying the intricate mechanisms of SIGLEC10 and its implications in various disease processes.
Product Name:
Human SIGLEC10 (Sialic Acid Binding Ig Like Lectin 10) CLIA Kit
SKU:
HUES00763
Target:
Human SIGLEC10 (Sialic Acid Binding Ig Like Lectin 10)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIGLEC10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIGLEC10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SIGLEC10. You can calculate the concentration of Human SIGLEC10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-114
102-118
88-100
Average (%)
107
108
95
1:4
Range (%)
92-104
86-99
103-115
Average (%)
99
91
109
1:8
Range (%)
88-100
85-98
93-103
Average (%)
93
91
98
1:16
Range (%)
100-119
91-101
94-112
Average (%)
108
96
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-112
106
EDTA plasma (n=5)
86-99
93
Cell culture media (n=5)
97-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.27
262.01
713.42
102.29
244.63
697.67
Standard deviation
10.13
23.66
50.94
11.68
27.25
77.86
C V (%)
10.75
9.03
7.14
11.42
11.14
11.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SIGLEC10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SIGLEC10 in samples. No significant cross-reactivity or interference between Human SIGLEC10 and analogues was observed.
