Human SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) ELISA Kit
The Human SIGLEC3 (Sialic acid-binding Ig-like lectin 3) ELISA Kit is specially designed for the precise measurement of SIGLEC3 levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring dependable and consistent results for various research purposes.SIGLEC3 is a key protein involved in immune responses and cell signaling. It plays a crucial role in regulating inflammatory processes and immune cell activation.
Dysregulation of SIGLEC3 has been linked to autoimmune diseases, cancer, and infectious diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Human SIGLEC3 ELISA Kit provides researchers with a powerful tool for studying the role of SIGLEC3 in various physiological and pathological processes, offering valuable insights for potential therapeutic interventions.
Product Name:
Human SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) ELISA Kit
SKU:
HUES02954
Target:
Human SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIGLEC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIGLEC3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SIGLEC3. You can calculate the concentration of Human SIGLEC3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
85-97
91-108
Average (%)
92
91
98
1:4
Range (%)
92-107
82-93
85-96
Average (%)
98
87
91
1:8
Range (%)
88-99
86-101
86-100
Average (%)
93
92
91
1:16
Range (%)
90-105
86-101
84-97
Average (%)
97
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
90
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.15
3.85
0.54
1.04
4.09
Standard deviation
0.04
0.06
0.2
0.03
0.06
0.19
C V (%)
7.41
5.22
5.19
5.56
5.77
4.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SIGLEC3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SIGLEC3 in samples. No significant cross-reactivity or interference between Human SIGLEC3 and analogues was observed.
