Human SIGLEC8 (Sialic Acid Binding Ig Like Lectin 8) CLIA Kit
The Human Siglec-8 (Sialic Acid Binding Ig-like Lectin 8) CLIA Kit is a reliable and sensitive assay designed for the accurate detection of Siglec-8 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and reproducible results for various research applications.Siglec-8 is a critical protein involved in immune responses, particularly in regulating inflammatory and allergic reactions.
Dysregulation of Siglec-8 has been associated with asthma, allergic disorders, and other inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human Siglec-8 CLIA Kit is a valuable tool for researchers looking to explore the role of Siglec-8 in immune responses and inflammatory diseases, providing accurate and reliable data for their studies.
Product Name:
Human SIGLEC8 (Sialic Acid Binding Ig Like Lectin 8) CLIA Kit
SKU:
HUES00766
Target:
Human SIGLEC8 (Sialic Acid Binding Ig Like Lectin 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIGLEC8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIGLEC8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SIGLEC8. You can calculate the concentration of Human SIGLEC8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
96-112
102-118
Average (%)
102
102
108
1:4
Range (%)
93-109
103-119
96-113
Average (%)
99
109
104
1:8
Range (%)
93-107
100-113
96-112
Average (%)
98
105
102
1:16
Range (%)
97-114
97-112
103-118
Average (%)
105
102
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-106
101
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
94-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
185.11
414.94
1622.13
190.03
452.56
1515.54
Standard deviation
16.4
46.31
98.95
21.8
43.67
104.42
C V (%)
8.86
11.16
6.1
11.47
9.65
6.89
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SIGLEC8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SIGLEC8 in samples. No significant cross-reactivity or interference between Human SIGLEC8 and analogues was observed.
