Human Signal Regulatory Protein Gamma (SIRPg) ELISA Kit
The Human Signal Regulatory Protein Gamma (SIRPG) ELISA Kit is a reliable tool for the accurate detection of SIRPG levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for a variety of research applications.SIRPG is a key protein involved in regulating immune responses and cell signaling processes. Its dysregulation has been linked to autoimmune diseases, inflammatory disorders, and cancer progression, highlighting its importance as a potential biomarker for disease diagnosis and therapeutic development.
By using the Human SIRPG ELISA Kit, researchers can gain valuable insights into the role of SIRPG in various disease conditions and explore its potential as a target for novel treatment strategies. Trust in the accuracy and reliability of this kit to advance your research efforts in immunology and beyond.
Product Name:
Human Signal Regulatory Protein Gamma (SIRPg) ELISA Kit
Product Code:
HUDL02686
Size:
96 Assays
Target:
SIRPg
Synonyms:
CD172g, CD172-G, SIRP-G, SIRP-B2, SIRPB2, SIRP-b2, SIRP-beta-2, SIRPgamma, CD172 antigen-like family member B, Signal-regulatory protein beta-2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
