SynonymsIPCA1,PCANAP1,PUMPCn,STAMP1,STMP,Prostate Cancer Associated Protein 1,Protein up-regulated in metastatic prostate cancer,SixTransMembrane protein of prostate 1
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
HUDL02793
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8NFT2
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
IPCA1,PCANAP1,PUMPCn,STAMP1,STMP,Prostate Cancer Associated Protein 1,Protein up-regulated in metastatic prostate cancer,SixTransMembrane protein of prostate 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit
The Human Six-Transmembrane Epithelial Antigen of the Prostate 2 (STEAP2) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of STEAP2 levels in human serum, plasma, and cell culture supernatants. This kit ensures accurate and reproducible results, making it ideal for a wide range of research applications.STEAP2 is a transmembrane protein that plays a critical role in cellular iron homeostasis and has been implicated in various diseases, including cancer and metabolic disorders.
Its expression levels can provide valuable insights into disease progression and treatment responses, making it an important biomarker for studying these conditions.With its high sensitivity and specificity, the STEAP2 ELISA Kit offers researchers a reliable tool for studying the role of STEAP2 in disease pathogenesis and developing potential therapeutic interventions. Order your kit today and unlock the potential of STEAP2 research.
Product Name:
Human Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2) ELISA Kit
Product Code:
HUDL02793
Size:
96 Assays
Target:
STEAP2
Synonyms:
IPCA1, PCANAP1, PUMPCn, STAMP1, STMP, Prostate Cancer Associated Protein 1, Protein up-regulated in metastatic prostate cancer, SixTransMembrane protein of prostate 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
