The Human PSA (Prostate-Specific Antigen) ELISA Kit is specifically designed for the precise detection of PSA levels in human serum, plasma, and cell culture supernatants. With a high level of sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for various research applications.PSA is a protein that is primarily produced by prostate gland cells and is commonly used as a biomarker for prostate cancer screening and monitoring. Elevated levels of PSA can indicate the presence of prostate cancer or other prostate-related conditions, highlighting the importance of reliable PSA testing. This ELISA kit provides researchers with a valuable tool for studying prostate cancer, monitoring disease progression, and evaluating treatment effectiveness. With its ease of use and robust performance, the Human PSA ELISA Kit from Assay Genie is an essential resource for advancing research in the field of prostate cancer and improving patient outcomes.
Product Name:
Human PSA (Prostate Specific Antigen) ELISA Kit
SKU:
HUES01377
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
