Human SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit
The Human SPA (Pulmonary Surfactant-associated Protein A) ELISA Kit is a reliable and accurate tool for detecting levels of SPA in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.SPA is a key protein involved in pulmonary surfactant function, essential for maintaining normal lung function and preventing respiratory distress syndrome.
Dysregulation of SPA levels has been associated with various pulmonary diseases, making it a valuable biomarker for studying and developing therapies for respiratory conditions.With its advanced technology and proven performance, the Human SPA ELISA Kit is a valuable asset for researchers studying lung biology, respiratory diseases, and therapeutic interventions. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human SPA (Pulmonary Surfactant Associated Protein A) ELISA Kit
SKU:
HUES02324
Target:
Human SPA (Pulmonary Surfactant Associated Protein A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SP-A1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SP-A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SP-A1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SP-A1. You can calculate the concentration of Human SP-A1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
97-109
92-105
Average (%)
100
102
98
1:4
Range (%)
87-100
85-96
83-94
Average (%)
93
90
89
1:8
Range (%)
91-103
83-96
86-96
Average (%)
96
89
91
1:16
Range (%)
85-99
82-93
84-96
Average (%)
92
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.86
136.08
414.87
48.73
132.09
424.02
Standard deviation
2.6
7.61
16.18
2.93
5.76
20.01
C V (%)
5.01
5.59
3.9
6.01
4.36
4.72
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SP-A1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SP-A1 in samples. No significant cross-reactivity or interference between Human SP-A1 and analogues was observed.
