The Human SPARCL1 (SPARC-like protein 1) CLIA Kit is specifically designed for the accurate and reliable detection of SPARCL1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SPARCL1, also known as Hevin, is a critical protein involved in cell adhesion, migration, and tissue remodeling. It plays a key role in various physiological and pathological processes, such as wound healing, neural development, and cancer progression.
The measurement of SPARCL1 levels can provide valuable insights into these processes and may serve as a potential biomarker for disease diagnosis and therapeutic development.Overall, the Human SPARCL1 CLIA Kit from Assay Genie is an essential tool for researchers studying the role of SPARCL1 in different biological pathways and diseases, offering accurate and efficient detection of this important protein.
Product Name:
Human SPARCL1 (SPARC Like Protein 1) CLIA Kit
SKU:
HUES00729
Target:
Human SPARCL1 (SPARC Like Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SPARCL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPARCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPARCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SPARCL1. You can calculate the concentration of Human SPARCL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
99-111
95-109
Average (%)
101
104
103
1:4
Range (%)
85-100
90-103
87-103
Average (%)
91
95
94
1:8
Range (%)
84-96
87-101
91-105
Average (%)
91
94
96
1:16
Range (%)
93-107
101-117
90-104
Average (%)
100
108
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-115
106
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
92-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.73
553.13
1740.74
182.8
540.3
1831.46
Standard deviation
21.08
60.01
105.31
18.72
59.32
149.81
C V (%)
10.66
10.85
6.05
10.24
10.98
8.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SPARCL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SPARCL1 in samples. No significant cross-reactivity or interference between Human SPARCL1 and analogues was observed.
