Human SPT betaN4 (Spectrin Beta, Non Erythrocytic 4) CLIA Kit
The Human SPT-BETA/N4 (Spectrin beta non-erythrocytic 4) CLIA Kit is a highly specific and sensitive assay designed for the accurate detection of SPT-BETA/N4 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for researchers in various fields.SPT-BETA/N4 is a critical protein involved in various cellular processes, including cell shape and organization, membrane stability, and intracellular signaling.
Dysregulation of SPT-BETA/N4 has been linked to a variety of diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.With its high sensitivity and specificity, the Human SPT-BETA/N4 CLIA Kit offers researchers a powerful tool for accurately measuring SPT-BETA/N4 levels, aiding in the advancement of scientific research and potential therapeutic opportunities.
Product Name:
Human SPT betaN4 (Spectrin Beta, Non Erythrocytic 4) CLIA Kit
SKU:
HUES01253
Target:
Human SPT betaN4 (Spectrin Beta, Non Erythrocytic 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SPTβN4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPTβN4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPTβN4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SPTβN4. You can calculate the concentration of Human SPTβN4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
101-114
90-106
Average (%)
92
108
97
1:4
Range (%)
92-103
94-108
85-101
Average (%)
97
99
92
1:8
Range (%)
95-108
89-102
97-113
Average (%)
101
96
103
1:16
Range (%)
102-116
89-101
101-114
Average (%)
108
94
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
105
EDTA plasma (n=5)
91-102
97
Cell culture media (n=5)
99-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.8
245.6
988.16
93.35
260.81
940.49
Standard deviation
11.41
19.26
81.33
10.62
27.88
95.18
C V (%)
11.55
7.84
8.23
11.38
10.69
10.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SPTβN4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SPTβN4 in samples. No significant cross-reactivity or interference between Human SPTβN4 and analogues was observed.
