The Human SPX (Spexin) ELISA Kit is specifically designed for the quantification of SPX levels in human serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, providing accurate and consistent results for various research applications.SPX, also known as spexin, is a novel peptide hormone that regulates various physiological processes including metabolism, appetite, and stress response.
It has been implicated in conditions such as obesity, diabetes, and cardiovascular diseases, making it a valuable biomarker for studying these diseases and developing potential treatments.With its easy-to-use format and reliable performance, the Human SPX ELISA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of SPX in human health and disease.
Product Name:
Human SPX (Spexin) ELISA Kit
SKU:
HUES03578
Target:
Human SPX (Spexin) ELISA Kit
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SPX. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SPX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SPX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SPX. You can calculate the concentration of Human SPX in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-100
91-106
86-97
Average (%)
94
97
92
1:4
Range (%)
92-105
81-91
83-96
Average (%)
97
86
88
1:8
Range (%)
88-101
84-97
88-104
Average (%)
93
91
95
1:16
Range (%)
86-101
81-93
88-103
Average (%)
92
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
101
EDTA plasma (n=5)
94-107
99
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
252.13
716.26
1809.17
272.69
679.36
1914.47
Standard deviation
13.87
40.61
92.63
15.24
29.96
79.83
C V (%)
5.5
5.67
5.12
5.59
4.41
4.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SPX concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SPX in samples. No significant cross-reactivity or interference between Human SPX and analogues was observed.
