Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit
The Human sRANKL (Soluble Receptor Activator of Nuclear Factor Kb Ligand) ELISA Kit is a powerful tool for the precise measurement of sRANKL levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research purposes.sRANKL is a vital protein involved in bone metabolism, regulating osteoclast differentiation and function. Dysregulation of sRANKL signaling is associated with bone-related diseases such as osteoporosis and rheumatoid arthritis, highlighting the importance of studying sRANKL levels in various pathological conditions.
By using the Human sRANKL ELISA Kit, researchers can gain valuable insights into the role of sRANKL in bone health and disease, paving the way for the development of targeted therapies and diagnostic tools. With its advanced technology and reliable performance, this kit is an indispensable asset for investigating sRANKL biology and its implications in clinical settings.
Product Name:
Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) ELISA Kit
SKU:
HUES03534
Target:
Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sRANKL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sRANKL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sRANKL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sRANKL. You can calculate the concentration of Human sRANKL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
94-106
83-98
Average (%)
103
100
90
1:4
Range (%)
86-97
83-97
85-99
Average (%)
92
89
92
1:8
Range (%)
93-109
79-94
86-96
Average (%)
99
86
91
1:16
Range (%)
88-103
81-94
85-100
Average (%)
95
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
99
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
92-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.28
122.42
372.33
55.3
121.01
370.3
Standard deviation
3.2
5.66
17.61
3.65
5.14
16.11
C V (%)
6.12
4.62
4.73
6.6
4.25
4.35
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sRANKL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sRANKL in samples. No significant cross-reactivity or interference between Human sRANKL and analogues was observed.
