Human RANkappaL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit
The RANKL ELISA Kit is meticulously designed for the precise quantitative measurement of Receptor Activator of Nuclear Factor Kappa B Ligand (RANKL) levels in diverse human biological samples. RANKL, a key regulator of bone metabolism, immune responses, and inflammatory processes, plays a pivotal role in bone resorption, remodeling, and immune-cell differentiation. Understanding the precise levels of RANKL is critical in unraveling its pathophysiological roles and its implications in inflammatory conditions and bone disorders.
Our RANKL ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible outcomes. Crafted under stringent quality standards, this kit guarantees robust performance and user-friendly protocols suitable for both research and clinical applications. Count on our RANKL ELISA Kit for the reliable and precise quantification of this vital biomarker to advance your studies and investigations effectively.
Product Name:
Human RANkappaL (Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit
SKU:
AEES00042
Target:
Human RANκL (Receptor Activator of Nuclear Factor Kappa B Ligand)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RANκL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RANκL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RANκL. You can calculate the concentration of Human RANκL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
93-102
84-99
Average (%)
102
98
92
1:4
Range (%)
87-94
101-113
83-98
Average (%)
91
105
91
1:8
Range (%)
95-102
91-102
89-102
Average (%)
98
95
96
1:16
Range (%)
94-101
96-107
96-104
Average (%)
98
104
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
87-95
92
Cell culture media (n=5)
93-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.74
107.45
474.3
41.26
113.95
481.22
Standard deviation
2.43
5.29
25.19
2.32
5.44
22.86
C V (%)
5.82
4.92
5.31
5.62
4.77
4.75
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human RANκL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human RANκL in samples. No significant cross-reactivity or interference between Human RANκL and analogues was observed.
