Human suPAR (Soluble Urokinase-type Plasminogen Activator Receptor) ELISA Kit
The suPAR ELISA Kit is designed for the quantitative detection of Soluble Urokinase-type Plasminogen Activator Receptor (suPAR) levels in various human biological samples.
suPAR is a critical biomarker involved in the regulation of immune and inflammatory responses, including cell adhesion, migration, and tissue remodeling. Its measurement can provide insights into various pathological states including infection, inflammation, and malignancy, making it an important target for disease monitoring and therapeutic intervention. Assay Genie's suPAR ELISA Kit offers superior sensitivity and specificity, ensuring reliable and reproducible results. Produced under strict quality control standards, this kit provides strong performance and is user-friendly, making it an excellent choice for both research and clinical applications. Trust Assay Genie's suPAR ELISA Kit for accurate and dependable quantification of this important biomarker in your studies.
Product Name:
Human suPAR (Soluble Urokinase-type Plasminogen Activator Receptor) ELISA Kit
SKU:
AEES00035
Target:
Human suPAR (Soluble Urokinase-type Plasminogen Activator Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human suPAR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human suPAR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human suPAR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human suPAR. You can calculate the concentration of Human suPAR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
87-101
94-110
Average (%)
105
94
102
1:4
Range (%)
87-100
84-97
87-101
Average (%)
94
90
92
1:8
Range (%)
88-101
80-92
83-96
Average (%)
93
86
90
1:16
Range (%)
93-107
84-96
88-100
Average (%)
98
89
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
92-104
97
Cell culture media (n=5)
96-107
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.35
3.71
0.48
1.43
3.49
Standard deviation
0.03
0.06
0.2
0.03
0.06
0.15
C V (%)
5.88
4.44
5.39
6.25
4.2
4.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human suPAR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human suPAR in samples. No significant cross-reactivity or interference between Human suPAR and analogues was observed.
