Human Surfactant Associated Protein D (SPD) ELISA Kit
The Human Surfactant-Associated Protein D (SP-D) ELISA Kit is a powerful tool for the precise detection of surfactant-associated protein D levels in human serum, plasma, and cell culture samples. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable resource for various research applications.Surfactant-associated protein D is a key player in the immune defense system of the lungs, playing a critical role in the clearance of pathogens and maintenance of pulmonary homeostasis. Dysregulation of SP-D has been linked to respiratory disorders such as asthma, chronic obstructive pulmonary disease (COPD), and interstitial lung diseases, highlighting its significance as a biomarker for studying and developing treatments for these conditions.
Whether investigating respiratory diseases, studying lung function, or exploring immune responses, the Human Surfactant-Associated Protein D (SP-D) ELISA Kit provides researchers with a reliable and accurate method for measuring SP-D levels, advancing their understanding of pulmonary health and disease.
Product Name:
Human Surfactant Associated Protein D (SPD) ELISA Kit
Product Code:
HUDL02753
Size:
96 Assays
Target:
SPD
Synonyms:
SFTPD, COLEC7, PSP-D, SFTP4, SP-D, Pulmonary Surfactant Protein D, Collectin-7, Lung surfactant protein D
Detection Method:
Sandwich
Range:
6.25-400ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
