Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
The Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit is specifically designed for the precise quantification of SPD levels in human samples, including serum, plasma, and cell culture supernatants. This highly sensitive and specific kit ensures accurate and consistent results, making it well-suited for various research applications.SPD is a key protein involved in pulmonary surfactant function, playing a crucial role in maintaining lung integrity and function. Changes in SPD levels have been associated with various respiratory disorders, making it a valuable biomarker for studying lung diseases and developing potential therapeutic interventions.Overall, the Human SPD ELISA Kit provides researchers with a reliable tool for investigating the role of SPD in pulmonary health and disease, offering valuable insights for advancing our understanding and treatment of respiratory conditions.
Product Name:
Human SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit
SKU:
HUES02327
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
