The Human Synpo (Synaptopodin) CLIA Kit is specifically designed for the precise measurement of synaptopodin levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Synaptopodin is a key protein involved in the regulation of synaptic plasticity and dendritic spine morphology in the brain. Dysregulation of synaptopodin has been implicated in various neurological disorders, including Alzheimer's disease and schizophrenia.
Therefore, the Human Synpo CLIA Kit is an essential tool for studying the role of synaptopodin in these conditions and exploring potential therapeutic interventions.Overall, the Human Synpo (Synaptopodin) CLIA Kit is a valuable resource for researchers seeking to investigate the function and significance of synaptopodin in health and disease. Its reliability and precision make it a top choice for studies focused on neurobiology and neurodegenerative disorders.
Product Name:
Human SYNPO (Synaptopodin) CLIA Kit
SKU:
HUES01089
Target:
Human SYNPO (Synaptopodin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SYNPO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SYNPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SYNPO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SYNPO. You can calculate the concentration of Human SYNPO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
87-98
88-98
Average (%)
102
93
93
1:4
Range (%)
100-117
88-100
90-104
Average (%)
107
94
97
1:8
Range (%)
85-99
85-98
85-97
Average (%)
92
90
91
1:16
Range (%)
95-110
100-114
96-109
Average (%)
102
108
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
105
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.03
170.04
869.89
106.51
169.09
869.7
Standard deviation
11.99
19.52
65.94
13.62
19.09
66.1
C V (%)
11.1
11.48
7.58
12.79
11.29
7.6
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SYNPO concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SYNPO in samples. No significant cross-reactivity or interference between Human SYNPO and analogues was observed.
