Human TAFI (Thrombin Activatable Fibrinolysis Inhibitor) CLIA Kit
The Human TAFI (Thrombin-activatable Fibrinolysis Inhibitor) CLIA Kit is a cutting-edge assay designed for the precise measurement of TAFI levels in human samples such as serum, plasma, and cell culture supernatants. With unparalleled accuracy and sensitivity, this kit delivers dependable and consistent results, making it an indispensable tool for a diverse array of research applications.TAFI is a vital player in fibrinolysis regulation, modulating the breakdown of blood clots and maintaining hemostasis. Dysregulation of TAFI has been implicated in various disease states, including thrombotic disorders, cardiovascular conditions, and inflammatory diseases.
By accurately measuring TAFI levels, researchers can gain valuable insights into these pathologies and potentially identify novel therapeutic targets.Overall, the Human TAFI CLIA Kit offers an advanced solution for studying TAFI biology and its implications in disease, providing researchers with the tools they need to drive innovation and progress in the field of biomedical research.
Product Name:
Human TAFI (Thrombin Activatable Fibrinolysis Inhibitor) CLIA Kit
SKU:
HUES01125
Target:
Human TAFI (Thrombin Activatable Fibrinolysis Inhibitor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.56 ng/mL
Detection range:
0.94-60 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TAFI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TAFI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TAFI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TAFI. You can calculate the concentration of Human TAFI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-116
93-109
91-104
Average (%)
110
101
98
1:4
Range (%)
88-100
92-103
101-117
Average (%)
94
97
107
1:8
Range (%)
89-103
97-110
96-109
Average (%)
96
104
102
1:16
Range (%)
94-111
95-110
88-99
Average (%)
102
101
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-111
105
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.76
6.35
26.06
2.5
6.17
23.9
Standard deviation
0.22
0.68
1.96
0.3
0.71
1.85
C V (%)
7.97
10.71
7.52
12.0
11.51
7.74
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TAFI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TAFI in samples. No significant cross-reactivity or interference between Human TAFI and analogues was observed.
