Human TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit
The Human TAFI (Thrombin-Activatable Fibrinolysis Inhibitor) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of TAFI levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for a wide range of research applications.TAFI is a key regulator of fibrinolysis, controlling the breakdown of blood clots and maintaining hemostasis.
Dysregulation of TAFI activity has been implicated in various diseases, including thrombosis, cardiovascular disorders, and inflammatory conditions. The Human TAFI ELISA Kit provides researchers with a valuable tool for studying TAFI biology and exploring its role in disease pathogenesis, potentially leading to the development of novel diagnostic and therapeutic strategies.
Product Name:
Human TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit
SKU:
HUES03016
Target:
Human TAFI (Thrombin Activatable Fibrinolysis Inhibitor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.00 ng/mL
Detection range:
5.00-320 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TAFI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TAFI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TAFI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TAFI. You can calculate the concentration of Human TAFI in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
-
84-99
-
Average (%)
-
91
-
1:4
Range (%)
-
86-97
-
Average (%)
-
92
-
1:8
Range (%)
-
83-93
-
Average (%)
-
88
-
1:16
Range (%)
-
85-98
-
Average (%)
-
90
-
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
-
-
EDTA plasma (n=5)
94-110
100
Cell culture media (n=5)
-
-
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
14.87
49.02
140.43
13.61
44.71
139.44
Standard deviation
0.83
2.18
5.5
0.74
1.87
7.33
C V (%)
5.58
4.45
3.92
5.44
4.18
5.26
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TAFI concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TAFI in samples. No significant cross-reactivity or interference between Human TAFI and analogues was observed.
