Human TARC (Thymus Activation Regulated Chemokine) CLIA Kit
The Human TARC (Thymus Activation Regulated Chemokine) CLIA Kit is specifically designed for the precise measurement of TARC levels in human samples, including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent choice for various research applications.TARC is a key chemokine responsible for regulating immune cell migration, particularly in conditions like asthma, atopic dermatitis, and other inflammatory disorders.
By detecting TARC levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify novel therapeutic targets.Overall, the Human TARC CLIA Kit is a valuable tool for researchers seeking to explore the role of TARC in various diseases and develop targeted treatments. Its reliable performance and user-friendly design make it a must-have for laboratories conducting research in immunology and inflammatory diseases.
Product Name:
Human TARC (Thymus Activation Regulated Chemokine) CLIA Kit
SKU:
HUES00026
Target:
Human TARC (Thymus Activation Regulated Chemokine)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TARC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TARC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TARC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TARC. You can calculate the concentration of Human TARC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
97-111
97-115
Average (%)
96
103
105
1:4
Range (%)
93-106
91-108
94-107
Average (%)
98
99
101
1:8
Range (%)
98-114
100-119
88-99
Average (%)
105
108
94
1:16
Range (%)
89-102
102-113
93-106
Average (%)
96
107
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
98
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
91-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.32
106.81
382.44
39.32
109.52
411.93
Standard deviation
3.5
7.88
29.95
3.86
8.62
38.89
C V (%)
8.47
7.38
7.83
9.82
7.87
9.44
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TARC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TARC in samples. No significant cross-reactivity or interference between Human TARC and analogues was observed.
