Human TDGF1 (Teratocarcinoma Derived Growth Factor 1) CLIA Kit
The Human TDGF1 (Teratocarcinoma-Derived Growth Factor 1) CLIA Kit is specifically designed for the precise measurement of TDGF1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.TDGF1, also known as Cripto, is a critical growth factor involved in embryonic development, stem cell maintenance, and cancer progression.
Its role in promoting cell growth, differentiation, and survival makes it a valuable target for studying various diseases and exploring therapeutic interventions.This CLIA kit provides researchers with a reliable tool to investigate the role of TDGF1 in disease pathology and potential treatment strategies. With its high-quality performance and ease of use, the Human TDGF1 CLIA Kit is an essential resource for advancing scientific knowledge and medical advancements.
Product Name:
Human TDGF1 (Teratocarcinoma Derived Growth Factor 1) CLIA Kit
SKU:
HUES00836
Target:
Human TDGF1 (Teratocarcinoma Derived Growth Factor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TDGF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TDGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TDGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TDGF1. You can calculate the concentration of Human TDGF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-106
88-98
93-107
Average (%)
100
93
100
1:4
Range (%)
94-108
86-100
97-110
Average (%)
100
92
104
1:8
Range (%)
88-103
103-118
88-101
Average (%)
94
110
94
1:16
Range (%)
93-107
89-102
99-115
Average (%)
99
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-97
91
EDTA plasma (n=5)
96-110
102
Cell culture media (n=5)
91-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
101.64
248.5
736.1
96.2
234.7
793.69
Standard deviation
12.14
29.07
76.7
11.08
17.77
50.56
C V (%)
11.94
11.7
10.42
11.52
7.57
6.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TDGF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TDGF1 in samples. No significant cross-reactivity or interference between Human TDGF1 and analogues was observed.
