Human TERT (Telomerase Reverse Transcriptase) CLIA Kit
The Human TERT (Telomerase Reverse Transcriptase) CLIA Kit is specifically designed for the quantitative detection of TERT levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TERT is a key enzyme in telomerase, responsible for maintaining telomere length and playing a crucial role in cellular immortalization and aging processes. Aberrant TERT expression has been associated with aging, cancer, and other diseases, making it an important biomarker for understanding these conditions and potentially developing therapeutic interventions.
By utilizing the Human TERT CLIA Kit, researchers can accurately measure TERT levels in human samples, allowing for valuable insights into the role of telomerase in various biological processes and diseases. This kit provides the necessary tools for advancing research in areas related to telomere maintenance, aging, and cancer biology.
Product Name:
Human TERT (Telomerase Reverse Transcriptase) CLIA Kit
SKU:
HUES00451
Target:
Human TERT (Telomerase Reverse Transcriptase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TERT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TERT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TERT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TERT. You can calculate the concentration of Human TERT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-115
87-102
94-108
Average (%)
105
94
99
1:4
Range (%)
88-102
85-98
99-112
Average (%)
96
92
105
1:8
Range (%)
94-110
94-109
85-100
Average (%)
102
100
92
1:16
Range (%)
93-107
90-105
89-104
Average (%)
99
97
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-100
92
EDTA plasma (n=5)
100-116
107
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.57
199.74
885.55
96.04
209.98
898.19
Standard deviation
13.02
18.06
70.76
10.25
21.65
76.26
C V (%)
12.33
9.04
7.99
10.67
10.31
8.49
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TERT concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human TERT in samples. No significant cross-reactivity or interference between Human TERT and analogues was observed.
