The Human TF (Tissue Factor) CLIA Kit is a cutting-edge assay designed for the precise measurement of tissue factor levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Tissue factor, also known as thromboplastin, is a key protein involved in the blood clotting process. Dysregulation of tissue factor has been linked to various diseases like blood clotting disorders, cardiovascular diseases, and inflammation.
Monitoring tissue factor levels using this kit can provide valuable insights into the mechanisms underlying these conditions and facilitate the development of potential therapeutic strategies.This advanced CLIA kit from Assay Genie is user-friendly and delivers rapid results, making it an indispensable tool for researchers and clinicians investigating the role of tissue factor in health and disease. Order now to unlock the potential of tissue factor research with the Human TF CLIA Kit.
Product Name:
Human TF (Tissue Factor) CLIA Kit
SKU:
HUES00039
Target:
Human TF (Tissue Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TF. You can calculate the concentration of Human TF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
96-107
86-99
Average (%)
94
102
92
1:4
Range (%)
103-117
88-100
95-109
Average (%)
108
94
101
1:8
Range (%)
101-116
88-100
86-100
Average (%)
107
94
91
1:16
Range (%)
88-98
97-112
93-107
Average (%)
93
103
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-115
108
EDTA plasma (n=5)
92-109
100
Cell culture media (n=5)
95-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.81
52.44
218.76
23.18
52.3
231.75
Standard deviation
2.58
5.68
19.49
2.71
5.47
25.4
C V (%)
10.84
10.83
8.91
11.69
10.46
10.96
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TF in samples. No significant cross-reactivity or interference between Human TF and analogues was observed.
