The Human TF (Tissue Factor) ELISA Kit is specifically designed for the quantitative measurement of tissue factor levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and dependable results for a variety of research purposes.Tissue factor is a key protein involved in blood coagulation and inflammation, playing a crucial role in various physiological and pathological processes. Dysregulation of tissue factor has been linked to conditions such as thrombosis, inflammation, and cancer, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.With its easy-to-use format and reliable performance, the Human TF ELISA Kit is an essential tool for researchers seeking to investigate the role of tissue factor in health and disease. Order yours today and take your research to the next level.
Product Name:
Human TF (Tissue Factor) ELISA Kit
SKU:
HUES01327
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
