Human TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit
The Human TFF3 (Trefoil Factor 3) Intestinal ELISA Kit is specifically designed for the quantitative detection of TFF3 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and accurate, ensuring precise and consistent results for a variety of research applications.TFF3, also known as trefoil factor 3, is a key protein involved in mucosal protection and repair in the gastrointestinal tract. It plays a crucial role in maintaining the integrity of the intestinal lining and promoting healing of damaged tissue. Dysregulation of TFF3 levels has been associated with digestive disorders such as inflammatory bowel disease (IBD) and gastric ulcers, making it a valuable biomarker for studying these conditions and developing potential treatments.
With its advanced technology and proven performance, the Human TFF3 Intestinal ELISA Kit from Assay Genie is a valuable tool for researchers studying the role of TFF3 in gastrointestinal health and disease. Order yours today to advance your research and make impactful discoveries in the field of gastrointestinal biology.
Product Name:
Human TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit
SKU:
HUES02200
Target:
Human TFF3 (Trefoil Factor 3, Intestinal )
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TFF3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TFF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TFF3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TFF3. You can calculate the concentration of Human TFF3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
92-109
96-108
Average (%)
103
99
103
1:4
Range (%)
86-101
79-92
86-99
Average (%)
93
85
91
1:8
Range (%)
89-105
87-99
90-103
Average (%)
96
92
95
1:16
Range (%)
89-101
80-93
84-97
Average (%)
94
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
95-110
100
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
262.49
596.57
1684.46
237.33
645.36
1752.3
Standard deviation
13.96
28.99
61.31
13.55
31.62
90.94
C V (%)
5.32
4.86
3.64
5.71
4.9
5.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TFF3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TFF3 in samples. No significant cross-reactivity or interference between Human TFF3 and analogues was observed.
