Human TGF beta I (Transforming Growth Factor Beta Induced Protein) ELISA Kit
The Human TGF-beta I (Transforming Growth Factor Beta-Induced Protein) ELISA Kit is specially designed to accurately measure TGF-beta I levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.TGF-beta I is a vital protein that plays a key role in regulating cell growth, differentiation, and immune response. It is involved in numerous biological processes, including embryonic development, tissue repair, and immune modulation.
Dysregulation of TGF-beta signaling has been linked to various diseases such as cancer, fibrosis, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.Overall, the Human TGF-beta I ELISA Kit provides researchers with a powerful tool to accurately quantify TGF-beta I levels in human samples, facilitating in-depth studies on its role in health and disease.
Product Name:
Human TGF beta I (Transforming Growth Factor Beta Induced Protein) ELISA Kit
SKU:
HUES02657
Target:
Human TGF beta I (Transforming Growth Factor Beta Induced Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGFβI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGFβI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGFβI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TGFβI. You can calculate the concentration of Human TGFβI in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
92-107
95-110
Average (%)
106
97
103
1:4
Range (%)
92-104
83-97
87-97
Average (%)
97
88
92
1:8
Range (%)
90-105
82-94
87-102
Average (%)
97
87
93
1:16
Range (%)
90-104
80-91
88-102
Average (%)
96
85
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-97
92
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
88-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.09
4.11
14.96
2.13
4.18
14.94
Standard deviation
0.14
0.22
0.7
0.14
0.18
0.67
C V (%)
6.7
5.35
4.68
6.57
4.31
4.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TGFβI concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGFβI in samples. No significant cross-reactivity or interference between Human TGFβI and analogues was observed.
