Human TGF- beta1 (Transforming Growth Factor Beta 1) CLIA Kit
The Human TGF-Beta 1 (Transforming Growth Factor Beta 1) CLIA Kit is specifically designed for the precise measurement of TGF-Beta 1 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.TGF-Beta 1 is a key regulatory protein involved in various cellular processes, including cell growth, differentiation, and immune response modulation.
Dysregulation of TGF-Beta 1 has been implicated in numerous diseases such as cancer, fibrosis, and autoimmune disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its user-friendly protocol and high-quality components, the Human TGF-Beta 1 CLIA Kit from Assay Genie offers researchers a valuable tool for investigating the role of TGF-Beta 1 in disease pathogenesis and exploring new treatment strategies.
Product Name:
Human TGF- beta1 (Transforming Growth Factor Beta 1) CLIA Kit
SKU:
HUES00106
Target:
Human TGF- beta1 (Transforming Growth Factor Beta 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-β1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TGF-β1. You can calculate the concentration of Human TGF-β1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
100-112
90-102
Average (%)
93
107
95
1:4
Range (%)
91-107
87-99
95-110
Average (%)
99
92
102
1:8
Range (%)
97-111
88-104
87-100
Average (%)
104
95
92
1:16
Range (%)
96-109
87-100
94-107
Average (%)
104
92
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-116
106
EDTA plasma (n=5)
99-114
107
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.67
126.2
268.56
41.64
127.35
261.43
Standard deviation
4.89
12.62
18.07
3.81
10.54
20.05
C V (%)
11.46
10.0
6.73
9.15
8.28
7.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TGF-β1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TGF-β1 in samples. No significant cross-reactivity or interference between Human TGF-β1 and analogues was observed.
