The Human TJP1 (Tight Junction Protein 1) CLIA Kit is specifically designed for the precise measurement of TJP1 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for a variety of research applications.TJP1, also known as ZO-1, is a key component of tight junctions, crucial for maintaining the integrity and barrier function of epithelial and endothelial cells. Dysregulation of TJP1 has been implicated in various diseases, including inflammatory disorders, autoimmune conditions, and cancer.
By measuring TJP1 levels, researchers can gain insights into the role of tight junctions in disease pathology and potential therapeutic interventions.Overall, the Human TJP1 CLIA Kit provides researchers with a reliable and efficient method for studying the role of TJP1 in various disease states, paving the way for new discoveries and advancements in the field of biomedical research.
Product Name:
Human TJP1 (Tight Junction Protein 1) CLIA Kit
SKU:
HUES00862
Target:
Human TJP1 (Tight Junction Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TJP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TJP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TJP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TJP1. You can calculate the concentration of Human TJP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
103-119
93-104
Average (%)
103
110
98
1:4
Range (%)
99-112
92-105
96-112
Average (%)
104
100
103
1:8
Range (%)
93-110
102-115
104-121
Average (%)
100
109
110
1:16
Range (%)
95-111
86-99
99-112
Average (%)
103
91
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
102
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
92-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.68
208.3
937.4
90.75
189.42
1028.95
Standard deviation
7.64
20.68
63.56
11.57
16.76
83.45
C V (%)
8.33
9.93
6.78
12.75
8.85
8.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TJP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TJP1 in samples. No significant cross-reactivity or interference between Human TJP1 and analogues was observed.
