Human TJP1 (Tight junction protein ZO-1) ELISA Kit
The Human TJP1 (Tight Junction Protein ZO-1) ELISA Kit is specifically designed for the accurate measurement of TJP1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TJP1, also known as ZO-1, is a key protein involved in maintaining the integrity of tight junctions between cells. These tight junctions play a critical role in regulating the passage of molecules and ions across cell barriers, making TJP1 essential for proper cell function.
Dysregulation of TJP1 has been implicated in various diseases, including inflammatory disorders, autoimmune diseases, and cancer, highlighting its significance as a potential biomarker for studying and developing therapies for these conditions.Overall, the Human TJP1 ELISA Kit provides researchers with a reliable tool for studying the role of TJP1 in various physiological and pathological processes, ultimately contributing to a better understanding of tight junction biology and its implications for human health.
Product Name:
Human TJP1 (Tight junction protein ZO-1) ELISA Kit
SKU:
HUES02538
Target:
Human TJP1 (Tight junction protein ZO-1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TJP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TJP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TJP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TJP1. You can calculate the concentration of Human TJP1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
90-102
98-112
Average (%)
91
96
104
1:4
Range (%)
88-100
82-94
88-99
Average (%)
94
88
94
1:8
Range (%)
93-106
84-98
82-94
Average (%)
99
90
87
1:16
Range (%)
85-100
82-95
84-98
Average (%)
92
88
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
96-108
101
Cell culture media (n=5)
90-107
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.29
4.4
0.55
1.17
4.57
Standard deviation
0.03
0.06
0.24
0.03
0.05
0.14
C V (%)
6
4.65
5.45
5.45
4.27
3.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TJP1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TJP1 in samples. No significant cross-reactivity or interference between Human TJP1 and analogues was observed.
