The Human TLR2 (Toll-like Receptor 2) CLIA Kit is a cutting-edge assay designed for the accurate quantification of TLR2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers unmatched sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.TLR2 is a key player in the innate immune response, recognizing and responding to microbial pathogens.
Dysregulation of TLR2 has been implicated in various inflammatory and infectious diseases, making it a valuable biomarker for studying immune responses and developing targeted therapies.With its advanced technology and ease of use, the Human TLR2 CLIA Kit is an indispensable tool for researchers seeking to unravel the complexities of the immune system and investigate potential therapeutic interventions.
Product Name:
Human TLR2 (Toll-Like Receptor 2) CLIA Kit
SKU:
HUES00566
Target:
Human TLR2 (Toll-Like Receptor 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TLR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TLR2. You can calculate the concentration of Human TLR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
97-112
91-105
Average (%)
104
103
99
1:4
Range (%)
104-116
94-110
96-112
Average (%)
110
102
103
1:8
Range (%)
92-104
89-103
95-113
Average (%)
99
94
103
1:16
Range (%)
89-103
99-117
92-109
Average (%)
97
107
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
106
EDTA plasma (n=5)
98-116
106
Cell culture media (n=5)
94-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.61
560.41
1772.46
204.13
592.31
1738.18
Standard deviation
21.47
46.4
141.62
22.86
60.12
114.2
C V (%)
10.81
8.28
7.99
11.2
10.15
6.57
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TLR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TLR2 in samples. No significant cross-reactivity or interference between Human TLR2 and analogues was observed.
