The Human TLR2 (Toll-Like Receptor 2) ELISA Kit is specifically designed for the accurate detection of TLR2 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.TLR2 is a key protein involved in the innate immune response, recognizing pathogens and activating the immune system. Dysregulation of TLR2 has been implicated in inflammatory diseases, autoimmune disorders, and infectious diseases, highlighting its importance as a biomarker in studying these conditions and developing targeted therapies.
Overall, the Human TLR2 ELISA Kit from Assaygenie provides researchers with a powerful tool to investigate the role of TLR2 in health and disease, offering valuable insights into immune responses and potential therapeutic interventions.
Product Name:
Human TLR2 (Toll-Like Receptor 2) ELISA Kit
SKU:
HUES02074
Target:
Human TLR2 (Toll-Like Receptor 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TLR-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TLR-2. You can calculate the concentration of Human TLR-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
91-103
90-105
Average (%)
95
97
97
1:4
Range (%)
88-104
87-99
86-97
Average (%)
96
92
91
1:8
Range (%)
93-105
82-94
89-102
Average (%)
99
86
94
1:16
Range (%)
94-105
85-97
84-94
Average (%)
100
92
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-105
96
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
90-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.03
1.83
8.0
1.11
1.76
7.92
Standard deviation
0.05
0.07
0.39
0.07
0.08
0.24
C V (%)
4.85
3.83
4.88
6.31
4.55
3.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TLR-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TLR-2 in samples. No significant cross-reactivity or interference between Human TLR-2 and analogues was observed.
