Human TNF- beta (Tumor Necrosis Factor Beta) CLIA Kit
The Human TNF-Beta (Tumor Necrosis Factor Beta) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of TNF-Beta levels in human serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an excellent choice for various research applications.TNF-Beta is a key cytokine involved in inflammation, immune response, and cell survival. Dysregulation of TNF-Beta has been implicated in a variety of diseases, including cancer, autoimmune disorders, and inflammatory conditions.
Understanding TNF-Beta levels can provide valuable insights into disease mechanisms and potential therapeutic targets.By utilizing the Human TNF-Beta CLIA Kit, researchers can gain valuable information on TNF-Beta levels in biological samples, leading to advancements in the study and treatment of various diseases. Trust in the accuracy and precision of this kit to support your research goals effectively.
Product Name:
Human TNF- beta (Tumor Necrosis Factor Beta) CLIA Kit
SKU:
HUES01198
Target:
Human TNF- beta (Tumor Necrosis Factor Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-β. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-β, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNF-β. You can calculate the concentration of Human TNF-β in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-111
84-95
92-103
Average (%)
102
90
97
1:4
Range (%)
97-109
93-109
90-101
Average (%)
102
100
95
1:8
Range (%)
101-116
90-106
89-103
Average (%)
108
97
95
1:16
Range (%)
95-110
84-97
101-113
Average (%)
102
91
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-113
106
EDTA plasma (n=5)
88-98
93
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.29
62.81
189.4
27.97
57.46
189.16
Standard deviation
2.74
4.89
15.99
2.61
6.03
17.93
C V (%)
10.42
7.79
8.44
9.33
10.49
9.48
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNF-β concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNF-β in samples. No significant cross-reactivity or interference between Human TNF-β and analogues was observed.
