Human TNF- beta (Tumor Necrosis Factor Beta) ELISA Kit
The Human TNF-Beta (Tumor Necrosis Factor-Beta) ELISA Kit is specially designed to accurately measure TNF-Beta levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring consistent and dependable results for a variety of research purposes.TNF-Beta is a key cytokine that plays a critical role in inflammation, immune response, and cell death. Dysregulation of TNF-Beta is implicated in various diseases including inflammatory disorders, autoimmune conditions, and cancer. Thus, monitoring TNF-Beta levels can provide valuable insights into disease progression and potentially guide therapeutic interventions.In summary, the Human TNF-Beta ELISA Kit offers researchers a powerful tool to investigate the role of TNF-Beta in health and disease, paving the way for innovative discoveries and potential treatment strategies.
Product Name:
Human TNF- beta (Tumor Necrosis Factor Beta) ELISA Kit
SKU:
HUES03183
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
