Human TNFSF14 (Tumor Necrosis Factor Ligand Superfamily, Member 14) CLIA Kit
The Human TNFSF14 (Tumor Necrosis Factor Ligand Superfamily Member 14) CLIA Kit is specifically designed for the precise measurement of TNFSF14 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.TNFSF14, also known as LIGHT (lymphotoxin-like inducible protein that competes with glycoprotein D for herpesvirus entry on T cells), is a key member of the tumor necrosis factor superfamily that plays a critical role in immune response modulation and inflammation regulation.
Dysregulation of TNFSF14 has been linked to various diseases, including autoimmune disorders and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its advanced technology and reliability, the Human TNFSF14 CLIA Kit is an invaluable tool for researchers and healthcare professionals seeking to understand the role of TNFSF14 in disease pathogenesis and progression.
Product Name:
Human TNFSF14 (Tumor Necrosis Factor Ligand Superfamily, Member 14) CLIA Kit
SKU:
HUES00091
Target:
Human TNFSF14 (Tumor Necrosis Factor Ligand Superfamily, Member 14)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNFSF14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNFSF14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNFSF14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNFSF14. You can calculate the concentration of Human TNFSF14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
91-103
96-111
Average (%)
106
97
101
1:4
Range (%)
92-103
91-107
100-118
Average (%)
98
99
108
1:8
Range (%)
85-98
89-102
93-106
Average (%)
91
95
99
1:16
Range (%)
96-109
89-101
97-110
Average (%)
103
95
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
93
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
43.42
78.25
311.28
43.07
75.8
315.52
Standard deviation
5.31
7.9
23.69
4.67
6.24
21.77
C V (%)
12.23
10.1
7.61
10.84
8.23
6.9
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNFSF14 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNFSF14 in samples. No significant cross-reactivity or interference between Human TNFSF14 and analogues was observed.
