Human TNNI2 (Troponin I Type 2, Fast Skeletal) CLIA Kit
The Human TNNT2 (Troponin I Type 2, fast skeletal) CLIA Kit is a reliable and accurate assay for the detection of TNNT2 levels in human serum, plasma, and tissue samples. This kit boasts high sensitivity and specificity, allowing for precise and reproducible results in a variety of research settings.TNNT2 is an important protein found in fast skeletal muscle tissue, playing a key role in muscle contraction and function. Abnormal levels of TNNT2 have been linked to various muscular disorders, making it a valuable biomarker for studying muscle-related diseases and potential therapeutic interventions.
Whether you are researching muscle physiology, studying muscular diseases, or exploring potential treatments, the Human TNNT2 CLIA Kit is an essential tool for your laboratory. Trust Assay Genie for accurate and reliable results in your research endeavors.
Product Name:
Human TNNI2 (Troponin I Type 2, Fast Skeletal) CLIA Kit
SKU:
HUES00834
Target:
Human TNNI2 (Troponin I Type 2, Fast Skeletal)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TNNI2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNNI2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNNI2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TNNI2. You can calculate the concentration of Human TNNI2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
91-106
87-101
Average (%)
104
99
92
1:4
Range (%)
94-105
98-114
98-117
Average (%)
100
104
106
1:8
Range (%)
89-101
92-104
89-103
Average (%)
95
98
95
1:16
Range (%)
94-109
96-108
99-116
Average (%)
100
103
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
93-108
101
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.99
87.5
482.53
45.81
89.87
493.61
Standard deviation
5.0
6.28
50.13
4.29
8.7
55.83
C V (%)
10.87
7.18
10.39
9.36
9.68
11.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TNNI2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNNI2 in samples. No significant cross-reactivity or interference between Human TNNI2 and analogues was observed.
