The Human TPO (Thrombopoietin) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of thrombopoietin levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for various research applications.Thrombopoietin is a key regulator of platelet production and is essential for normal blood clotting. Dysregulation of thrombopoietin levels has been associated with various hematological disorders such as thrombocytopenia and myeloproliferative neoplasms.
Therefore, accurate measurement of thrombopoietin levels is crucial for understanding these conditions and developing targeted therapies.The Human TPO CLIA Kit offers researchers a valuable tool for studying the role of thrombopoietin in health and disease, providing insights into its potential as a therapeutic target. With its high sensitivity and specificity, this kit is a reliable choice for researchers working in the fields of hematology, oncology, and immunology.
Product Name:
Human TPO (Thrombopoietin) CLIA Kit
SKU:
HUES00904
Target:
Human TPO (Thrombopoietin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TPO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TPO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TPO. You can calculate the concentration of Human TPO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-115
87-98
87-100
Average (%)
107
93
94
1:4
Range (%)
87-103
91-101
94-108
Average (%)
94
96
100
1:8
Range (%)
98-110
101-115
95-107
Average (%)
104
108
102
1:16
Range (%)
91-104
103-120
94-110
Average (%)
96
110
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
94
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
95-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.87
87.91
370.41
38.21
95.78
370.24
Standard deviation
3.97
7.11
30.67
4.28
8.97
29.55
C V (%)
9.96
8.09
8.28
11.2
9.37
7.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TPO in samples. No significant cross-reactivity or interference between Human TPO and analogues was observed.
