Human Trefoil Factor 3, Intestinal (TFF3) ELISA Kit
The Human Trefoil Factor 3 (Intestinal TFF3) ELISA Kit is specifically designed for the accurate detection of TFF3 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring consistent and reliable results for a variety of research applications.TFF3 is a key protein involved in maintaining the integrity of the intestinal mucosa and protecting against gastrointestinal injury and inflammation. It plays a crucial role in conditions such as inflammatory bowel disease, colorectal cancer, and mucosal healing, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
Our Human Trefoil Factor 3 ELISA Kit offers researchers a powerful tool for studying the role of TFF3 in various physiological and pathological conditions, providing valuable insights into disease mechanisms and potential treatment strategies. Trust Assay Genie for high-quality ELISA kits that deliver accurate and reproducible results for your research needs.
Product Name:
Human Trefoil Factor 3, Intestinal (TFF3) ELISA Kit
Product Code:
HUDL02861
Size:
96 Assays
Target:
TFF3
Synonyms:
HITF, ITF3, TFI, HP1.B
Detection Method:
Sandwich
Range:
125-8,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
