The Human TSP-4 (Thrombospondin-4) CLIA Kit is specifically designed for the precise quantification of thrombospondin-4 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.Thrombospondin-4 is a vital protein known for its role in cell-matrix interactions, tissue remodeling, and wound healing. Dysregulation of thrombospondin-4 has been associated with various diseases, including cancer, cardiovascular disorders, and inflammatory conditions.
Therefore, this CLIA kit serves as a valuable tool for investigating these pathologies and identifying potential therapeutic targets.Explore the intricate world of thrombospondin-4 with our high-quality CLIA kit, unravelling its biological significance and unlocking new insights into disease mechanisms and treatment strategies.
Product Name:
Human TSP-4 (Thrombospondin-4) CLIA Kit
SKU:
HUES00977
Target:
Human TSP-4 (Thrombospondin-4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human TSP-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TSP-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TSP-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human TSP-4. You can calculate the concentration of Human TSP-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
99-113
101-116
Average (%)
107
107
107
1:4
Range (%)
87-101
97-115
93-109
Average (%)
93
105
99
1:8
Range (%)
93-107
88-103
103-119
Average (%)
98
95
109
1:16
Range (%)
96-109
92-106
91-105
Average (%)
104
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
102
EDTA plasma (n=5)
98-112
104
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.3
265.54
856.0
99.21
252.98
833.04
Standard deviation
10.23
29.08
68.05
12.28
18.21
84.39
C V (%)
9.36
10.95
7.95
12.38
7.2
10.13
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human TSP-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TSP-4 in samples. No significant cross-reactivity or interference between Human TSP-4 and analogues was observed.
