Human Ubiquitin Conjugating Enzyme E2C (UBE2C) ELISA Kit
The Human Ubiquitin Conjugating Enzyme E2C (UBE2C) ELISA Kit is a reliable tool for the quantitative measurement of UBE2C levels in human serum, plasma, and cell culture supernatants. This kit is specifically designed for high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.UBE2C is a key enzyme involved in the ubiquitination process, playing a crucial role in protein degradation and regulating various cellular processes.
Dysregulation of UBE2C has been linked to cancer, neurodegenerative diseases, and other disorders, highlighting its importance as a potential biomarker for studying these conditions and developing new therapies.Overall, the Human UBE2C ELISA Kit offers researchers a valuable tool for investigating the role of UBE2C in health and disease, providing essential insights into its functions and potential therapeutic targets.
Product Name:
Human Ubiquitin Conjugating Enzyme E2C (UBE2C) ELISA Kit
Product Code:
HUDL03032
Size:
96 Assays
Target:
UBE2C
Synonyms:
UBCH10, Ubiquitin carrier protein C, Ubiquitin-protein ligase C
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
